Stem cell research holds enormous promise for the understanding of basic biological processes and the treatment of diseases. Committing to it in a concerted effort is a powerful way to propel the fields of science and medicine forward.
The facility offers two highly demanded core services in human IPS reprogramming & human and mouse genome targeting. Further, the collaborative effort between IMBA and other Austrian academic/ clinical establishments has enabled the generation of a human IPS biobank, which contains quality reprogrammed healthy and disease lines that are accessible for all scientists. Additionally, HAPLOBANK is embedded within the facility, which is a repository and distributor of a genome-wide library of murine ESC lines with defined genetic mutations. Training and knowledge dissemination to the next generation of scientists is a core mission of the facility and annual courses or individual training upon request are being regularly offered.
Genome Targeting Service
We perform genome-editing in ESc/IPSc in Human and Mouse species.
TYPE OF EDITING
- Electroporation (AMAXA 4D system)
CRISPR delivery can be DNA or RNP based.
Time: Turnaround time depends on project complexity, but typically it ranges between 8-14 weeks.
Screening and final edited products: We do not perform genotyping on picked clones due to capacity limitations. Instead, internal costumers are tasked for this step. Once successful editing has been identified, the facility will proceed to expand 3-5 edited clones and hand them over as frozen vials. These clones will be also banked in our facility.
REQUEST A GENOME TARGETING SERVICE
To submit a request for Genome Targeting in JIRA: https://jira.imp.ac.at/servicedesk/customer/portal/4; click on Gene Targeting.
If you wish to provide us with your own stem cell line and targeting reagents please attach relevant handling and QC information in the JIRA submission form. Provision of good quality starting material is solely the responsibility of the costumer.
Human IPSC Reprogramming Service
TYPE OF REPROGRAMMING TECHNOLOGY AND BIOMATERIAL USED
We offer IMBA groups Sendai Virus non-integrating reprogramming technologies under feeder-free conditions to reprogram from healthy and diseased source material:
- Blood (Peripheral Blood Mononuclear Cells (PBMCs).
- Skin biopsies (fibroblasts)
Turnaround time ranges between 12-16 weeks, depending on complexity and disease background status. All lines are regularly checked for mycoplasma.
Upon request we can perform QC services as listed under ‘QUALITY CONTROL SERVICE OF HUMAN STEM CELLS.’
Please note, that the facility must reply with existing ethics laws. It is the customer’s responsibility to provide the relevant patient consent and ethics approval.
REQUEST A HUMAN IPSC REPROGRAMMING SERVICE
To submit a request for Genome Targeting in JIRA: https://jira.imp.ac.at/servicedesk/customer/portal/4; click on ‘Human Reprogramming’.
The IMBA iPSC Biobank, is a collection of induced pluripotent stem cell lines (iPSC lines) produced from skin (fibroblasts) and blood cells (PBMCs). The iPSC Biobank is integrated into the IMBA Stem Cell Core Facility. It stores and provides iPSC lines from clinically normal test subjects as a resource for scientific research.
The iPSC Biobank lines are highly characterized and authenticated using immunological, phenotypic, genotypic, and sterility-based analyses. SCCF scientists perform immunocytochemistry and quantitative analyses to verify the presence of the appropriate markers of pluripotency. To confirm the pluripotent status, the colony morphology and karyotype of the iPSCs are examined. Finally, the cells are authenticated by short tandem repeat (STR) and tested for microbial contamination as well as for the presence of human pathogens.
The resource is available to internal IMBA groups, academic groups, and commercial customers. The ethical guidelines of the iPSC Biobank are always based on prevailing laws and internal regulations. We are currently building a webshop for the purchasing of IPSC lines, which will go live in autumn 2022. In the interim, please contact jennifer.volz(at)imba.oeaw.ac.at for information and requests of iPSC Biobank lines.
For more specific information on the ethics approval process, please contact Ethics & Biosafety.
Quality Control Service of Human Stem Cells
Prolonged culture conditions and genetic manipulation of cells may expose cells to selection pressures and subsequently compromise cell fitness and stem cell properties. We recommend for researchers to test the quality of their lines routinely during their work.
Following QC and reagent services are available for internal IMBA groups:
- SNP array-based genotyping
- Pluripotency tests: Alkaline Phosphatase (AP) staining pluripotency test, Expression of markers of the undifferentiated state (IF or Flow cytometry analyses), Tri-lineage differentiation
- Cell line submission and banking
- Viral panel testing (CMV, EBV, HBV, HCV, HSV-1, HSV-2, HIV, VZV, HPV) is available from the MBS Facility.
- Home-made stem cell media (E8)
REQUEST A SERVICE IN QUALITY CONTROL IN HUMAN STEM CELLS:
To submit a request in JIRA: https://jira.imp.ac.at/servicedesk/customer/portal/4; click on ‘Quality Control’.
Training & Outreach
We use well-established protocols which we adapt to our service pipelines. However, a lot of projects can be challenging due to technical and/or biological reasons we cannot control, and that may cause the service to be paused or stopped entirely. Our goal is to make this process transparent and help you prepare before requesting one of our services. Here you will find information on the ISCCF conditions, as well as considerations for your project planning.
By using our services, you have read and agreed to the ISCCF Service Terms & Conditions.