Stem cell research holds enormous promise for the understanding of basic biological processes and the treatment of diseases. Committing to it in a concerted effort is a powerful way to propel the fields of science and medicine forward.
The facility offers two highly demanded core services in human IPS reprogramming & human and mouse genome targeting. Further, the collaborative effort between IMBA and other Austrian academic/ clinical establishments has enabled the generation of a human IPS biobank, which contains quality reprogrammed healthy and disease lines that are accessible for all scientists. Additionally, HAPLOBANK is embedded within the facility, which is a repository and distributor of a genome-wide library of murine ESC lines with defined genetic mutations. Training and knowledge dissemination to the next generation of scientists is a core mission of the facility and annual courses or individual training upon request are being regularly offered.
Genome Targeting Service
Our genome editing platform covers Human and Mouse species.
Type of Editing
- Point Mutation/ Repairs
- Endogenous Tagging/Reporters
- AAVS1/ROSA26 locus targeting
Time: Turnaround time depends on project complexity, but typically it ranges between 8-14 weeks.
- Screening and final edited products: We do not perform genotyping on picked clones due to capacity limitations. The individual scientist is tasked for this step. The facility will proceed to expand and hand over up to 3 edited clones (as frozen vials) identified by the scientist. These clones will be also banked in our facility.
- The plate of picked clones that was used to generate the duplicate DNA plate for screening, will also be handed back to the scientist, in case additional clones need to be expanded. At this point the project is considered closed.
Request a Genome Targeting Service:
To submit a request for Genome Targeting in JIRA: https://jira.imp.ac.at/servicedesk/customer/portal/4; click on Gene Targeting.
If you wish to provide us with your own stem cell line and targeting reagents please attach relevant handling and QC information in the JIRA submission form. Be aware that you are solely responsible for the quality of the reagents you are providing to the facility.
Human IPSC Reprogramming Service
We serve requests from internal IMBA groups, academic groups, and commercial customers. Please note, that the facility must reply with existing ethics laws. It is the customer’s responsibility to provide the relevant patient consent and ethics approval.
TYPE OF REPROGRAMMING TECHNOLOGY AND BIOMATERIAL USED:
- We offer Sendai Virus non-integrating reprogramming technologies under feeder-free conditions to reprogram from healthy and diseased source material:
- Blood (Peripheral Blood Mononuclear Cells (PBMCs).
- Skin biopsies (fibroblasts)
- Turnaround time ranges between 12-16 weeks, depending on complexity and disease background status.
- Upon request we can test pluripotency via Antibody staining of key pluripotency factors.
- All lines are regularly checked for mycoplasma.
REQUEST A HUMAN IPSC REPROGRAMMING SERVICE:
To submit a request for Genome Targeting in JIRA: https://jira.imp.ac.at/servicedesk/customer/portal/4; click on ‘Human Reprogramming’.
The IMBA iPSC Biobank, is a collection of induced pluripotent stem cell lines (iPSC lines) produced from skin (fibroblasts) and blood cells (PBMCs). The iPSC Biobank is integrated into the IMBA Stem Cell Core Facility. It stores and provides patient-specific iPSC lines from patients having monogenic or polygenic illnesses, as well as clinically normal test subjects as a resource for the scientific research of both common and rare diseases.
The iPSC Biobank lines are highly characterized and authenticated using immunological, phenotypic, genotypic, and sterility-based analyses. SCCF scientists perform immunocytochemistry and quantitative analyses to verify the presence of the appropriate markers of pluripotency. To confirm the pluripotent status, the colony morphology and karyotype of the iPSCs are examined. Finally, the cells are authenticated by short tandem repeat (STR) and tested for microbial contamination.
The resource is available to internal IMBA groups, academic groups, and commercial customers. The ethical guidelines of the iPSC Biobank are always based on prevailing laws and internal regulations. We are currently building a webshop for the purchasing of IPSC lines, which will go live in late 2021. In the interim, please contact jennifer.volz(at)imba.oeaw.ac.at for information and requests of iPSC Biobank lines.
For more specific information on the ethics approval process, please contact Ethics & Biosafety.
Quality Control Service of Human Stem Cells
Prolonged culture conditions and genetic manipulation of cells may expose cells to selection pressures and subsequently compromise cell fitness and stem cell properties. We recommend for researchers to test the quality of their lines routinely during their work.
Following QC and reagent services are available:
- SNP array-based genotyping
- Alkaline Phosphatase (AP) pluripotency test
- Immunofluorescence antibody (IFA) staining for and quantitation of pluripotency markers
- Viral panel testing (CMV, EBV, HBV, HCV, HSV-1, HSV-2, HIV, VZV, HPV)
- Cell line submission and banking
- Home-made stem cell media (E8)
REQUEST A SERVICE IN QUALITY CONTROL IN HUMAN STEM CELLS:
To submit a request in JIRA: https://jira.imp.ac.at/servicedesk/customer/portal/4; click on ‘Quality Control’.
In the post-genomic era, science is accumulating large datasets from high-throughput screening. Validation of hits from such screens still requires genetic testing. To this end, we created HAPLOBANK, a genome-wide library of murine ESC lines with defined genetic mutations. HAPLOBANK contains over 100,000 conditionally mutated and reversible mESC clones, targeting about 70% of the protein-coding genome (almost 17,000 genes). The resource is available to all scientists and represents the largest ever library of homozygous mutant embryonic stem cell lines to date. HAPLOBANK overcomes issues arising from clonal variability, because mutations can be repaired in single cells and at whole genome scale. In addition, the haploid genome of these embryonic stem cells enables reverse genetics. HAPLOBANK can be used to functionally validate predictions or to study in-depth phenotypic effects of gene loss, thereby helping to phenotypically annotate the mouse genome.
Training & Outreach
We offer hands-on training to researchers interested to learn stem cell handling. We organize courses and workshops and we engage with educators and students.
We are planning our next HANDS-ON HUMAN STEM CELL WORKSHOP, as soon as there are any updates available you will be able to find them here.
We use well-established protocols which we adapt to our service pipelines. However, a lot of projects can be challenging due to technical and/or biological reasons we cannot control, and that may cause the service to be paused or stopped entirely. Our goal is to make this process transparent and help you prepare before requesting one of our services. Here you will find information on the ISCCF conditions, as well as considerations for your project planning.
By using our services, you have read and agreed to the ISCCF Service Terms & Conditions.