16.01.2025

Unprecedented protein quantification in single cells using advanced proteomics

Scientists at the Proteomics Tech Hub of IMBA, the IMP and the GMI at the Vienna BioCenter recently developed a new single-cell proteomics workflow that enables an unprecedented level of protein quantification at the single-cell level.

The scientists, led by Julia Bubis and Manuel Matzinger at the Proteomics Tech Hub, collaborated with Thermo Fisher Scientific to develop a new workflow that takes advantage of the Astral mass spectrometer to deeply study the protein content of one cell at a time, allowing the researchers to find rare or unique cell types hidden within a larger group. The new workflow is particularly useful to study small systems with an inherent cellular heterogeneity. In collaboration with Nicolas Rivron’s group at IMBA, the researchers analyzed blastoids -a model of the early-stage embryo with just 100 cells and three cell lineages-, and were able to study the biology of each lineage in detail.

Read more: https://www.viennabiocenter.org/about/news/unprecedented-protein-quantification-in-single-cells-using-advanced-proteomics/