Tetsuya Hisanaga

Current projects:

Project 1: Chromatin dynamics during sexual reproduction

During sperm development in bryophytes, histone-based nucleosomes are replaced by sperm-specific nuclear basic proteins such as protamine. This means that chromatin landscapes encoded by histone post-translational modifications (PTMs) are erased during spermatogenesis and must be re-established after fertilization. I would like to understand mechanisms regulating this dynamic change in chromatin landscape during sexual reproduction using a model bryophyte, Marchantia polymorpha.

Project 2: Role of PRC2 in transposon silencing

Transposable elements (TEs) are mobile DNA fragments that can jump within a genome. Expression of TEs is usually silenced since TE expression and jumping could lead to genomic instability. In mammals and flowering plants, most TEs are packed and silenced by constitutive heterochromatin which is covered by H3K9me2/3 marks. Contrary to this, in Marchantia, we discovered that some TEs are covered by H3K27me3 deposited by PRC2 complex. I would like to address the question of whether these TE-associated H3K27me3 marks have a role in TE silencing.

Publications:

Cui, Y., Hisanaga, T., Kajiwara T., Yamaoka, S., Kohchi, T., Goh, T., and Nakajima, K. (2023) Three-Dimensional Morphological Analysis Revealed the Cell Patterning Bases for the Sexual Dimorphism Development in the Liverwort Marchantia Polymorpha. bioRxiv. https://doi.org/10.1101/2023.01.24.525312
Hisanaga, T., Romani, F., Wu, S., Kowar, T., Lintermann, R., Jamge, B., Montgomery, S. A., Axelsson, E., Dierschke, T., Bowman, J. L., Fujiwara, T., Hirooka, S., Miyagishima, S.-Y., Dolan, L., Schubert, D., & Berger, F. (2022). Transposons repressed by H3K27me3 were co-opted as cis-regulatory elements of H3K27me3 controlled protein coding genes during evolution of plants. In bioRxiv (p. 2022.10.24.513474). https://doi.org/10.1101/2022.10.24.513474
Hisanaga, T., Wu, S., Axelsson, E., Akimcheva, S., Dolan, L., & Berger, F. (2022). The chromatin landscape of bryophytes. In bioRxiv (p. 2022.10.21.513199). https://doi.org/10.1101/2022.10.21.513199
Montgomery, S. A., Hisanaga, T., Wang, N., Axelsson, E., Akimcheva, S., Sramek, M., Liu, C., & Berger, F. (2022). Polycomb-mediated repression of paternal chromosomes maintains haploid dosage in diploid embryos of Marchantia. eLife, 11. https://doi.org/10.7554/eLife.79258
Hisanaga, T., Fujimoto, S., Cui Y., Sato, K., Sano, R., Yamaoka, S., Kohchi, T., Berger, F., & Nakajima, K. (2021). Deep evolutionary origin of gamete-directed zygote activation by KNOX/BELL transcription factors in green plants. eLife,2021;10:e57090 DOI: 10.7554/eLife.57090　
Hisanaga, T., Okahashi, K., Yamaoka, S., Kajiwara, T., Nishihama, R., Shimamura, M., Yamato, K. T., Bowman, J. L., Kohchi, T., & Nakajima, K. (2019). A cis-acting bidirectional transcription switch controls sexual dimorphism in the liverwort. EMBO J, 38(6). DOI:10.15252/embj.2018100240
Nakamura, K., Hisanaga, T., Fujimoto, K., Nakajima, K., & Wada, H. (2018). Plant-inspired pipettes. Journal of the Royal Society Interface, 15(140). DOI:10.1098/rsif.2017.0868
Koi, S., Hisanaga, T., Sato, K., Shimamura, M., Yamato, K. T., Ishizaki, K., Kohchi, T., & Nakajima, K (2016). An Evolutionarily Conserved Plant RKD Factor Controls Germ Cell Differentiation. Curr Biol, 26(13), 1775-1781. DOI:10.1016/j.cub.2016.05.013
Hisanaga, T., Ferjani, A., Horiguchi, G., Ishikawa, N., Fujikura, U., Kubo, M., Demura, T., Fukuda, H., Ishida, T., Sugimoto, K., & Tsukaya, H. (2013). The ATM-dependent DNA damage response acts as an upstream trigger for compensation in the fas1 mutation during Arabidopsis leaf development. Plant Physiol, 162(2), 831-841. DOI:10.1104/pp.113.216796
Hisanaga, T., Yamaoka, S., Kawashima, T., Higo, A., Nakajima, K., Araki, T., Kohchi, T., & Berger, F. (2019). Building new insights in plant gametogenesis from an evolutionary perspective. Nature Plants, 5, 663–669. DOI: 10.1038/s41477-019-0466-0
Hisanaga, T., Kawade, K., & Tsukaya, H. (2015). Compensation: a key to clarifying the organ-level regulation of lateral organ size in plants. J Exp Bot, 66(4), 1055-1063. DOI:10.1093/jxb/erv028
Hisanaga, T., Miyashima, S., & Nakajima, K. (2014). Small RNAs as positional signal for pattern formation. Curr Opin Plant Biol, 21, 37-42. DOI:10.1016/j.pbi.2014.06.005

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Tetsuya Hisanaga

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